Generator
PL luxI

Part:BBa_K193001

Designed by: Shinya Tahara   Group: iGEM09_Tokyo_Tech   (2009-10-14)

CI repressible LuxI enzyme generator

CI repressible LuxI enzyme generator.

This part is used to transform E.coli strain which expresses CI represser.

We improved existing BioBrick Part BBa_F1610, and constructed K193001.

the vertical line is fluorescence intensity (a.u.)

luxR(AHL+),luxR(AHL-) ; strain N4830 transformed only with luxR expressing plasmid(BBa_J54140)

luxR+luxI; strain N4830 transformed with luxR expressing plasmid and luxI expressing plasmid

luxI; strain N4830 transformed with luxI expressing plasmid

More detail information, http://2009.igem.org/Team:Tokyo_Tech/Consortium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None